Cavite Isolation Of Microorganisms In Oil Pdf

Isolation and characterization of Lipolytic microorganisms

Isolation of Oil – Degrading Microorganisms in Spent

isolation of microorganisms in oil pdf

Microorganisms and Crude Oil InTech - Open. 2.1. Isolation of microorganisms from oil samples. Crude oil samples obtained from four wells (W-3, W-8, W-9 and W-11) of a Brazilian oil field were collected in sterile bottles. The temperature of the reservoir is about 40 В°C. Samples were stored at room temperature until use. For isolation of microorganisms, two different methods were used., ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF LIPASE PRODUCING MICROORGANISMS FROM ENVIRONMENT SHUBHAM VERMA1, KANTI PRAKASH SHARMA2 1Department of Microbiology, FASC, Mody University of Science and Technology, Lakshmangarh, Sikar - 332 311, Rajasthan, India..

Isolation and Characterization of Microorganisms from Oil

Isolation Identification and Characterization of Oil. The extraction of a microbial or single cell oil, for example comprising one or more polyunsaturated fatty acids (PUFAs), directly from microbial cells is disclosed which avoids the need for solvents. After fermentation, the microbial cells are pasteurised, washed and the cell walls lysed or disrupted by a mechanical (e.g. homogenisation, 2.1. Isolation of microorganisms from oil samples. Crude oil samples obtained from four wells (W-3, W-8, W-9 and W-11) of a Brazilian oil field were collected in sterile bottles. The temperature of the reservoir is about 40 В°C. Samples were stored at room temperature until use. For isolation of microorganisms, two different methods were used..

The use of microorganisms in remediating environmental contaminants such as crude oil sludge has become a promising technique owing to its economy and the fact it is environmentally friendly. Polycyclic aromatic hydrocarbons (PAHs), as the major components of oil sludge, are hydrophobic and recalcitrant. Oil contaminated Sea water, sediment and Soil sample (500g) collected from Oil Jetty, Paradip Port (Orissa) were used for isolation of hydrocarbon utilizing microorganisms. The samples were collected in pre-sterilized sample bottle following aseptic conditions. The samples duly …

Different agar media can be used for the isolation of Actinomycetes from soil sample after dilution of the soil slurry. Actinomycetes Isolation Agar is a selective medium commonly employed. It contains sodium caseinate as nitrogen source. Asparagine in addition to being an amino acid is also a source of nitrogen. ISOLATION AND SCREENING OF BIOSURFACTANT- PRODUCING BACTERIA FROM SOIL CONTAMINATED WITH DOMESTIC WASTE WATER. *Femi-Ola, T. O., Oluwole, O. A., Olowomofe, T. O. and Yakubu, H. Biosurfactant producing microorganisms were naturally present in oil contaminated soil.

PDF Screening of hydrocarbon degrading microorganisms from oil contaminated soil was studied and the samples were obtained from three Mechanical Workshops in Awka, Anambra State. The microorganisms were isolated using the pour plate method by selective enrichment technique. All... isolation, characterization and identification of microorganisms from waste dumping soils. Our results suggest that a number of microorganisms such as bacteria, fungi and Actinomycetes have the ability to degrade lignin, cellulose and hemicellulose content in these soils samples. MATERIALS AND METHODS

CHARACTERIZATION OF MICROORGANISMS IN INDOOR ENVIRONMENTS ACADEMIC DISSERTATION To be presented with the permission of the Faculty of Natural and Environmental Sciences, University of Kuopio, for public examination in auditorium L21, Snellmania Building, on February 9th 2008, at … Isolation, Identification and Characterization of Oil Degrading Bacteria Isolated 431 Biochemical characterization of selected microorganisms:- Biochemical tests viz; urease production, starch hydrolysis, carbohydrate fermentation (lactose and sucrose), catalase were performed with isolated hydrocarbon utilising bacteria (table no-2).

05.05.2015В В· Reduction of crystalline Fe(III) oxides is one of the most important electron sinks for organic compound oxidation in natural environments. Yet the limited number of isolates makes it difficult to understand the physiology and ecological impact of the microorganisms involved. Here, two-stage cultivation was implemented to selectively isolation of crude oil tolerant microorganisms and his capacity of degradation of crude oil in contaminated soils, such bacteria, yeast and fungi. Therefore, in this work, we isolate different microorganisms, which grow in presence of petroleum as the sole carbon source and we

09.11.2016В В· The use of microorganisms in remediating environmental contaminants such as crude oil sludge has become a promising technique owing to its economy and the fact it is environmentally friendly. Polycyclic aromatic hydrocarbons (PAHs), as the major components of oil sludge, are hydrophobic and recalcitrant. Isolation and identification of oil degrading bacteria from oil sludge in Abadan oil refinery Amin Afifi1, Hossein Motamedi1*, Bahram Alizadeh2, Hadi Leilavi1 1Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran 2Department of Geology, Faculty of Earth Science,Shahid Chamran University of Ahvaz, Ahvaz, Iran

Petroleum (crude) oil is complex mixture of many thousands of compounds. Petroleum hydrocarbons are the major constituents of crude oil (50-98%) and alkenes represent 20-50% of oil, depending on the source of the oil. Petroleum hydrocarbons can be degraded by microorganisms such as bacteria, fungi, yeast and microalgae. Oil contaminated Sea water, sediment and Soil sample (500g) collected from Oil Jetty, Paradip Port (Orissa) were used for isolation of hydrocarbon utilizing microorganisms. The samples were collected in pre-sterilized sample bottle following aseptic conditions. The samples duly …

09.11.2016В В· The use of microorganisms in remediating environmental contaminants such as crude oil sludge has become a promising technique owing to its economy and the fact it is environmentally friendly. Polycyclic aromatic hydrocarbons (PAHs), as the major components of oil sludge, are hydrophobic and recalcitrant. ISOLATION AND SCREENING OF BIOSURFACTANT- PRODUCING BACTERIA FROM SOIL CONTAMINATED WITH DOMESTIC WASTE WATER. *Femi-Ola, T. O., Oluwole, O. A., Olowomofe, T. O. and Yakubu, H. Biosurfactant producing microorganisms were naturally present in oil contaminated soil.

1. I.P COLLEGE,CAMPUS II A Presentation on topic Isolation and characterization of Microbes Submitted by- Meenu Sharma M.Sc. biotechnology (III SEM) 2. ISOLATION AND CHARACTERIZATION OF MICROBES WHAT ARE MICROBES? A microorganism or microbe is a microscopic living organism, which may be single-celled or multicellular. Isolation and characterization of cholesterol degrading bacteria from soap and vegetable oil industrial waste S Saranya1, S Shekinah1, T Rajagopal2, J Vijayakumar2 and P Ponmanickam1* 1Department of Microbiology and 2Department of Biotechnology, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi 626 124, India

1. I.P COLLEGE,CAMPUS II A Presentation on topic Isolation and characterization of Microbes Submitted by- Meenu Sharma M.Sc. biotechnology (III SEM) 2. ISOLATION AND CHARACTERIZATION OF MICROBES WHAT ARE MICROBES? A microorganism or microbe is a microscopic living organism, which may be single-celled or multicellular. The present investigation aims to isolate petroleum tolerant and degrading microbes from oil contaminated soil of oil refinery, which later be used in environmental biotechnology as remediating mechanisms. Bushnell Haas Media (BHM) supplemented with diesel oil as carbon and energy source, used for isolation of oil degrading bacteria.

MEOR offers major advantages over conventional EOR, namely low energy consumption and independence of the price of crude oil. In this work, the isolation and identification of microorganisms capable of producing biosurfactants and promote degradation of long-chain n-alkanes under conditions existent in oil reservoirs were addressed. 09.11.2016В В· The use of microorganisms in remediating environmental contaminants such as crude oil sludge has become a promising technique owing to its economy and the fact it is environmentally friendly. Polycyclic aromatic hydrocarbons (PAHs), as the major components of oil sludge, are hydrophobic and recalcitrant.

Oil contaminated Sea water, sediment and Soil sample (500g) collected from Oil Jetty, Paradip Port (Orissa) were used for isolation of hydrocarbon utilizing microorganisms. The samples were collected in pre-sterilized sample bottle following aseptic conditions. The samples duly … isolation of crude oil tolerant microorganisms and his capacity of degradation of crude oil in contaminated soils, such bacteria, yeast and fungi. Therefore, in this work, we isolate different microorganisms, which grow in presence of petroleum as the sole carbon source and we

CHARACTERIZATION OF MICROORGANISMS IN INDOOR ENVIRONMENTS ACADEMIC DISSERTATION To be presented with the permission of the Faculty of Natural and Environmental Sciences, University of Kuopio, for public examination in auditorium L21, Snellmania Building, on February 9th 2008, at … stimulates the isolation and selection of new strains [10]. Lipase-producing microorganisms have been found in different habitats such as industrial wastes, vegetable oil processing factories, dairy plants, and soil contaminated with oil and oilseeds among others. Lipases are produced by various microbes, such as

stimulates the isolation and selection of new strains [10]. Lipase-producing microorganisms have been found in different habitats such as industrial wastes, vegetable oil processing factories, dairy plants, and soil contaminated with oil and oilseeds among others. Lipases are produced by various microbes, such as pdf. Isolation and The population of microorganisms found in oil-polluted environment should degrade petroleum and higher rates than microorganisms in relatively clean environment [9]. we used soil and water samples from different oil contaminated sites for isolation and identification of petroleum degrading bacteria.

Isolation and Characterization of Bacteria Degrading Sumandak supplemented with two types of crude oil, either Sumandak or South Angsi at 1% (v/v) concentration. The optimum microorganisms such as bacteria, fungi and yeast (Chaillan et al. 2004). ADVERTISEMENTS: In this article we will discuss about the isolation and culture of microorganisms. Isolation of Microorganisms: Microorganisms occur in natural environment like soil. They are mixed with several other forms of life. Many microbes are pathogenic. They cause a number of diseases with a variety of symptoms, depending on how they

years of oil petroleum activity; in recent years this prob-lem has motivated researches to recover these contaminat-ed sites [2]. Microorganisms survive in contaminated habitat because they are metabolically capable of utilizing its resources and can occupy a suitable niche. Contaminants are often poten-tial energy sources for microorganisms [1]. This is to certify that the thesis entitled “ISOLATION AND CHARACTERIZATION OF PHENOL DEGRADING MICROORGANISMS FROM SOIL SAMPLE CONTAINING TRACES OF CRUDE OIL” submitted by Ms. Megha Saluja in partial fulfillment of the requirements for the grant of Bachelor of Technology Degree in Chemical Engineering at the

MEOR offers major advantages over conventional EOR, namely low energy consumption and independence of the price of crude oil. In this work, the isolation and identification of microorganisms capable of producing biosurfactants and promote degradation of long-chain n-alkanes under conditions existent in oil reservoirs were addressed. Isolation and identification of oil degrading bacteria from oil sludge in Abadan oil refinery Amin Afifi1, Hossein Motamedi1*, Bahram Alizadeh2, Hadi Leilavi1 1Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran 2Department of Geology, Faculty of Earth Science,Shahid Chamran University of Ahvaz, Ahvaz, Iran

Isolation, Optimization, and Molecular Characterization of a produced by microorganisms with the extracellular lipase being produced by many bacteria including Pseudomonas. The Keywords: KY288051, Oil contamination, Pseudomonas spp., 16S rRNA. Microorganisms and Crude Oil 115 Fig. 3. Structures of phen ol, furan, and cyclohexanol. Porphyrins often occur in crude oil. They are produc ts of degradation of dyes produced by living organisms. They are composed of pyrrole rings connected by methine bridges. They often form chelate compounds of nickel, vanadium, and other metals.

ISOLATION AND IDENTIFICATION OF BACTERIA AND FUNGI

isolation of microorganisms in oil pdf

Microorganisms Associated with Vegetable Oil Polluted Soil. Isolation of Microorganism from Natural Source for Bioremediation of Oil-spill *Susant Kumar Padhi 1, Alok kumar Meher2, Lopa pattanaik3, Srikant Tripathy1, BijyalaxmiBehera1 and Jasmin Mishra1 1Division of Biotechnology, MITS Engineering College, Rayagada, Odisha, 765017, Different agar media can be used for the isolation of Actinomycetes from soil sample after dilution of the soil slurry. Actinomycetes Isolation Agar is a selective medium commonly employed. It contains sodium caseinate as nitrogen source. Asparagine in addition to being an amino acid is also a source of nitrogen..

(PDF) Isolation and Enrichment of Microbes for Degradation. Isolation and screening of microorganisms from oil contaminated soil samples with biosurfactant producing ability was investigated. The biosurfactant producing ability of the microorganisms isolated from the soil samples was investigated by hemolytic assay, drop collapse test, emulsification index and methylene blue agar plate method. The best, Request PDF on ResearchGate Isolation and study of microorganisms from oil samples for application in Microbial Enhanced Oil Recovery Microbial Enhanced Oil Recovery (MEOR) is potentially useful to increment oil recovery from a reservoir beyond primary and secondary recovery operations using microorganisms and their metabolites. Stimulation.

Isolation Characterization and Identification of Diesel

isolation of microorganisms in oil pdf

Microorganisms and Crude Oil InTech - Open. Isolation, Identification and Characterization of Oil Degrading Bacteria Isolated 431 Biochemical characterization of selected microorganisms:- Biochemical tests viz; urease production, starch hydrolysis, carbohydrate fermentation (lactose and sucrose), catalase were performed with isolated hydrocarbon utilising bacteria (table no-2). Isolation of Microorganism from Natural Source for Bioremediation of Oil-spill *Susant Kumar Padhi 1, Alok kumar Meher2, Lopa pattanaik3, Srikant Tripathy1, BijyalaxmiBehera1 and Jasmin Mishra1 1Division of Biotechnology, MITS Engineering College, Rayagada, Odisha, 765017.

isolation of microorganisms in oil pdf


Isolation and screening of microorganisms from oil contaminated soil samples with biosurfactant producing ability was investigated. The biosurfactant producing ability of the microorganisms isolated from the soil samples was investigated by hemolytic assay, drop collapse test, emulsification index and methylene blue agar plate method. The best 1. I.P COLLEGE,CAMPUS II A Presentation on topic Isolation and characterization of Microbes Submitted by- Meenu Sharma M.Sc. biotechnology (III SEM) 2. ISOLATION AND CHARACTERIZATION OF MICROBES WHAT ARE MICROBES? A microorganism or microbe is a microscopic living organism, which may be single-celled or multicellular.

Different agar media can be used for the isolation of Actinomycetes from soil sample after dilution of the soil slurry. Actinomycetes Isolation Agar is a selective medium commonly employed. It contains sodium caseinate as nitrogen source. Asparagine in addition to being an amino acid is also a source of nitrogen. isolation of crude oil tolerant microorganisms and his capacity of degradation of crude oil in contaminated soils, such bacteria, yeast and fungi. Therefore, in this work, we isolate different microorganisms, which grow in presence of petroleum as the sole carbon source and we

09.11.2016В В· The use of microorganisms in remediating environmental contaminants such as crude oil sludge has become a promising technique owing to its economy and the fact it is environmentally friendly. Polycyclic aromatic hydrocarbons (PAHs), as the major components of oil sludge, are hydrophobic and recalcitrant. pdf. Isolation and The population of microorganisms found in oil-polluted environment should degrade petroleum and higher rates than microorganisms in relatively clean environment [9]. we used soil and water samples from different oil contaminated sites for isolation and identification of petroleum degrading bacteria.

Isolation, Optimization, and Molecular Characterization of a produced by microorganisms with the extracellular lipase being produced by many bacteria including Pseudomonas. The Keywords: KY288051, Oil contamination, Pseudomonas spp., 16S rRNA. Isolation of Microorganism from Natural Source for Bioremediation of Oil-spill *Susant Kumar Padhi 1, Alok kumar Meher2, Lopa pattanaik3, Srikant Tripathy1, BijyalaxmiBehera1 and Jasmin Mishra1 1Division of Biotechnology, MITS Engineering College, Rayagada, Odisha, 765017

1. I.P COLLEGE,CAMPUS II A Presentation on topic Isolation and characterization of Microbes Submitted by- Meenu Sharma M.Sc. biotechnology (III SEM) 2. ISOLATION AND CHARACTERIZATION OF MICROBES WHAT ARE MICROBES? A microorganism or microbe is a microscopic living organism, which may be single-celled or multicellular. 1. I.P COLLEGE,CAMPUS II A Presentation on topic Isolation and characterization of Microbes Submitted by- Meenu Sharma M.Sc. biotechnology (III SEM) 2. ISOLATION AND CHARACTERIZATION OF MICROBES WHAT ARE MICROBES? A microorganism or microbe is a microscopic living organism, which may be single-celled or multicellular.

microorganisms. The objective of this study was to isolate and characterize the biosurfactant producing bacteria from oil contaminated soil and study of their growth kinetics at different temperature and pH. The isolation and Growth study was carried out in MSM composition using kerosene oil as sole carbon source for bacterial growth. Isolation of Microorganism from Natural Source for Bioremediation of Oil-spill *Susant Kumar Padhi 1, Alok kumar Meher2, Lopa pattanaik3, Srikant Tripathy1, BijyalaxmiBehera1 and Jasmin Mishra1 1Division of Biotechnology, MITS Engineering College, Rayagada, Odisha, 765017

The extraction of a microbial or single cell oil, for example comprising one or more polyunsaturated fatty acids (PUFAs), directly from microbial cells is disclosed which avoids the need for solvents. After fermentation, the microbial cells are pasteurised, washed and the cell walls lysed or disrupted by a mechanical (e.g. homogenisation isolation, characterization and identification of microorganisms from waste dumping soils. Our results suggest that a number of microorganisms such as bacteria, fungi and Actinomycetes have the ability to degrade lignin, cellulose and hemicellulose content in these soils samples. MATERIALS AND METHODS

Different agar media can be used for the isolation of Actinomycetes from soil sample after dilution of the soil slurry. Actinomycetes Isolation Agar is a selective medium commonly employed. It contains sodium caseinate as nitrogen source. Asparagine in addition to being an amino acid is also a source of nitrogen. ISOLATION AND SCREENING OF BIOSURFACTANT- PRODUCING BACTERIA FROM SOIL CONTAMINATED WITH DOMESTIC WASTE WATER. *Femi-Ola, T. O., Oluwole, O. A., Olowomofe, T. O. and Yakubu, H. Biosurfactant producing microorganisms were naturally present in oil contaminated soil.

09.11.2016В В· The use of microorganisms in remediating environmental contaminants such as crude oil sludge has become a promising technique owing to its economy and the fact it is environmentally friendly. Polycyclic aromatic hydrocarbons (PAHs), as the major components of oil sludge, are hydrophobic and recalcitrant. The present investigation aims to isolate petroleum tolerant and degrading microbes from oil contaminated soil of oil refinery, which later be used in environmental biotechnology as remediating mechanisms. Bushnell Haas Media (BHM) supplemented with diesel oil as carbon and energy source, used for isolation of oil degrading bacteria.

isolation of microorganisms in oil pdf

Isolation and identification of oil degrading bacteria from oil sludge in Abadan oil refinery Amin Afifi1, Hossein Motamedi1*, Bahram Alizadeh2, Hadi Leilavi1 1Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran 2Department of Geology, Faculty of Earth Science,Shahid Chamran University of Ahvaz, Ahvaz, Iran Petroleum (crude) oil is complex mixture of many thousands of compounds. Petroleum hydrocarbons are the major constituents of crude oil (50-98%) and alkenes represent 20-50% of oil, depending on the source of the oil. Petroleum hydrocarbons can be degraded by microorganisms such as bacteria, fungi, yeast and microalgae.

ISOLATION AND SCREENING OF BIOSURFACTANT- PRODUCING

isolation of microorganisms in oil pdf

Isolation and characterization of cholesterol degrading. isolation of crude oil tolerant microorganisms and his capacity of degradation of crude oil in contaminated soils, such bacteria, yeast and fungi. Therefore, in this work, we isolate different microorganisms, which grow in presence of petroleum as the sole carbon source and we, MEOR offers major advantages over conventional EOR, namely low energy consumption and independence of the price of crude oil. In this work, the isolation and identification of microorganisms capable of producing biosurfactants and promote degradation of long-chain n-alkanes under conditions existent in oil reservoirs were addressed..

Isolation Optimization and Molecular Characterization of

ISOLATION OF SOIL BACTERIA FOR BIOREMEDIATION OF. ISOLATION AND SCREENING OF BIOSURFACTANT- PRODUCING BACTERIA FROM SOIL CONTAMINATED WITH DOMESTIC WASTE WATER. *Femi-Ola, T. O., Oluwole, O. A., Olowomofe, T. O. and Yakubu, H. Biosurfactant producing microorganisms were naturally present in oil contaminated soil., ISOLATION AND SCREENING OF BIOSURFACTANT- PRODUCING BACTERIA FROM SOIL CONTAMINATED WITH DOMESTIC WASTE WATER. *Femi-Ola, T. O., Oluwole, O. A., Olowomofe, T. O. and Yakubu, H. Biosurfactant producing microorganisms were naturally present in oil contaminated soil..

15.04.2015В В· Yet the diversity of the microorganisms involved in reduction of crystallized ferric irons and the mechanisms underlying the reduction process are largely unknown. A limited number of isolates makes it difficult to understand the physiology and ecological impact of the microorganisms. ADVERTISEMENTS: In this article we will discuss about the isolation and culture of microorganisms. Isolation of Microorganisms: Microorganisms occur in natural environment like soil. They are mixed with several other forms of life. Many microbes are pathogenic. They cause a number of diseases with a variety of symptoms, depending on how they

Different agar media can be used for the isolation of Actinomycetes from soil sample after dilution of the soil slurry. Actinomycetes Isolation Agar is a selective medium commonly employed. It contains sodium caseinate as nitrogen source. Asparagine in addition to being an amino acid is also a source of nitrogen. stimulates the isolation and selection of new strains [10]. Lipase-producing microorganisms have been found in different habitats such as industrial wastes, vegetable oil processing factories, dairy plants, and soil contaminated with oil and oilseeds among others. Lipases are produced by various microbes, such as

years of oil petroleum activity; in recent years this prob-lem has motivated researches to recover these contaminat-ed sites [2]. Microorganisms survive in contaminated habitat because they are metabolically capable of utilizing its resources and can occupy a suitable niche. Contaminants are often poten-tial energy sources for microorganisms [1]. Request PDF on ResearchGate Isolation and study of microorganisms from oil samples for application in Microbial Enhanced Oil Recovery Microbial Enhanced Oil Recovery (MEOR) is potentially useful to increment oil recovery from a reservoir beyond primary and secondary recovery operations using microorganisms and their metabolites. Stimulation

Title Isolation and characterization of bacteria from soil contaminated with diesel oil and the possible use of these in File Information WJMB23-12.pdf Hokkaido University Collection of Scholarly and Academic Papers : HUSCAP. microorganisms is unlikely to be acceptable to the public. Isolation and characterisation of crude oil sludge degrading bacteria Linda U. Obi1,3, Harrison I. Atagana2 and Rasheed A. Adeleke1,3,4* Background High demand for petroleum products all over the world has led to an increase in crude oil extraction and processing. This has resulted in an increased generation of large

05.05.2015 · Reduction of crystalline Fe(III) oxides is one of the most important electron sinks for organic compound oxidation in natural environments. Yet the limited number of isolates makes it difficult to understand the physiology and ecological impact of the microorganisms involved. Here, two-stage cultivation was implemented to selectively Oil contaminated Sea water, sediment and Soil sample (500g) collected from Oil Jetty, Paradip Port (Orissa) were used for isolation of hydrocarbon utilizing microorganisms. The samples were collected in pre-sterilized sample bottle following aseptic conditions. The samples duly …

Isolation and identification of oil degrading bacteria from oil sludge in Abadan oil refinery Amin Afifi1, Hossein Motamedi1*, Bahram Alizadeh2, Hadi Leilavi1 1Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran 2Department of Geology, Faculty of Earth Science,Shahid Chamran University of Ahvaz, Ahvaz, Iran The present investigation aims to isolate petroleum tolerant and degrading microbes from oil contaminated soil of oil refinery, which later be used in environmental biotechnology as remediating mechanisms. Bushnell Haas Media (BHM) supplemented with diesel oil as carbon and energy source, used for isolation of oil degrading bacteria.

The extraction of a microbial or single cell oil, for example comprising one or more polyunsaturated fatty acids (PUFAs), directly from microbial cells is disclosed which avoids the need for solvents. After fermentation, the microbial cells are pasteurised, washed and the cell walls lysed or disrupted by a mechanical (e.g. homogenisation Isolation and screening of microorganisms from oil contaminated soil samples with biosurfactant producing ability was investigated. The biosurfactant producing ability of the microorganisms isolated from the soil samples was investigated by hemolytic assay, drop collapse test, emulsification index and methylene blue agar plate method. The best

Microorganisms are therefore useful hi protecting the environment. It is sig-nificant to isolate microbes of high potential for the biodegradation of vegetable oil. Therefore, this study was undertaken to isolate, identify, characterize and screen microorganisms from vegetable oil contaminated soil samples. 2. Materials and Methods 2.1. Different agar media can be used for the isolation of Actinomycetes from soil sample after dilution of the soil slurry. Actinomycetes Isolation Agar is a selective medium commonly employed. It contains sodium caseinate as nitrogen source. Asparagine in addition to being an amino acid is also a source of nitrogen.

years of oil petroleum activity; in recent years this prob-lem has motivated researches to recover these contaminat-ed sites [2]. Microorganisms survive in contaminated habitat because they are metabolically capable of utilizing its resources and can occupy a suitable niche. Contaminants are often poten-tial energy sources for microorganisms [1]. 2.1. Isolation of microorganisms from oil samples. Crude oil samples obtained from four wells (W-3, W-8, W-9 and W-11) of a Brazilian oil field were collected in sterile bottles. The temperature of the reservoir is about 40 В°C. Samples were stored at room temperature until use. For isolation of microorganisms, two different methods were used.

ISOLATION AND SCREENING OF BIOSURFACTANT- PRODUCING BACTERIA FROM SOIL CONTAMINATED WITH DOMESTIC WASTE WATER. *Femi-Ola, T. O., Oluwole, O. A., Olowomofe, T. O. and Yakubu, H. Biosurfactant producing microorganisms were naturally present in oil contaminated soil. Isolation and characterisation of crude oil sludge degrading bacteria Linda U. Obi1,3, Harrison I. Atagana2 and Rasheed A. Adeleke1,3,4* Background High demand for petroleum products all over the world has led to an increase in crude oil extraction and processing. This has resulted in an increased generation of large

Isolation of Microorganism from Natural Source for Bioremediation of Oil-spill *Susant Kumar Padhi 1, Alok kumar Meher2, Lopa pattanaik3, Srikant Tripathy1, BijyalaxmiBehera1 and Jasmin Mishra1 1Division of Biotechnology, MITS Engineering College, Rayagada, Odisha, 765017 Isolation and characterisation of crude oil sludge degrading bacteria Linda U. Obi1,3, Harrison I. Atagana2 and Rasheed A. Adeleke1,3,4* Background High demand for petroleum products all over the world has led to an increase in crude oil extraction and processing. This has resulted in an increased generation of large

Isolation of Oil Degrading Microorganisms This was carried out using Minimal Salt Oil Agar. Aliquots (0.1ml) of soil sample suspension were inoculated on Minimal Salt Oil Agar by spread plate method and incubated at room temperature until colonies developed. The use of microorganisms in remediating environmental contaminants such as crude oil sludge has become a promising technique owing to its economy and the fact it is environmentally friendly. Polycyclic aromatic hydrocarbons (PAHs), as the major components of oil sludge, are hydrophobic and recalcitrant.

necessary to enumerate microorganisms, that is, to determine their numbers. It is also often essential to determine the number of microorganisms in a given sample. For example, the ability to determine the safety of many foods and drugs depends on knowing the levels of microorganisms … Isolation and characterization of cholesterol degrading bacteria from soap and vegetable oil industrial waste S Saranya1, S Shekinah1, T Rajagopal2, J Vijayakumar2 and P Ponmanickam1* 1Department of Microbiology and 2Department of Biotechnology, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi 626 124, India

05.05.2015В В· Reduction of crystalline Fe(III) oxides is one of the most important electron sinks for organic compound oxidation in natural environments. Yet the limited number of isolates makes it difficult to understand the physiology and ecological impact of the microorganisms involved. Here, two-stage cultivation was implemented to selectively Isolation and identification of oil degrading bacteria from oil sludge in Abadan oil refinery Amin Afifi1, Hossein Motamedi1*, Bahram Alizadeh2, Hadi Leilavi1 1Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran 2Department of Geology, Faculty of Earth Science,Shahid Chamran University of Ahvaz, Ahvaz, Iran

ADVERTISEMENTS: In this article we will discuss about the isolation and culture of microorganisms. Isolation of Microorganisms: Microorganisms occur in natural environment like soil. They are mixed with several other forms of life. Many microbes are pathogenic. They cause a number of diseases with a variety of symptoms, depending on how they 09.11.2016В В· The use of microorganisms in remediating environmental contaminants such as crude oil sludge has become a promising technique owing to its economy and the fact it is environmentally friendly. Polycyclic aromatic hydrocarbons (PAHs), as the major components of oil sludge, are hydrophobic and recalcitrant.

05.05.2015 · Reduction of crystalline Fe(III) oxides is one of the most important electron sinks for organic compound oxidation in natural environments. Yet the limited number of isolates makes it difficult to understand the physiology and ecological impact of the microorganisms involved. Here, two-stage cultivation was implemented to selectively Oil contaminated Sea water, sediment and Soil sample (500g) collected from Oil Jetty, Paradip Port (Orissa) were used for isolation of hydrocarbon utilizing microorganisms. The samples were collected in pre-sterilized sample bottle following aseptic conditions. The samples duly …

diesel oil broth and allowed to incubate at 37 oC for 1 week. Isolation of microorganisms: After 1 week of incubation period, 1 drop of enriched culture was spread on to the sterile modified diesel oil agar plate. The plate was incubated at 37 oC for 48 hr. After 48 hr incubation; two different bacterial Different agar media can be used for the isolation of Actinomycetes from soil sample after dilution of the soil slurry. Actinomycetes Isolation Agar is a selective medium commonly employed. It contains sodium caseinate as nitrogen source. Asparagine in addition to being an amino acid is also a source of nitrogen.

ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF LIPASE PRODUCING MICROORGANISMS FROM ENVIRONMENT SHUBHAM VERMA1, KANTI PRAKASH SHARMA2 1Department of Microbiology, FASC, Mody University of Science and Technology, Lakshmangarh, Sikar - 332 311, Rajasthan, India. microorganisms. The objective of this study was to isolate and characterize the biosurfactant producing bacteria from oil contaminated soil and study of their growth kinetics at different temperature and pH. The isolation and Growth study was carried out in MSM composition using kerosene oil as sole carbon source for bacterial growth.

Isolation and characterisation of crude oil sludge degrading bacteria Linda U. Obi1,3, Harrison I. Atagana2 and Rasheed A. Adeleke1,3,4* Background High demand for petroleum products all over the world has led to an increase in crude oil extraction and processing. This has resulted in an increased generation of large Isolation of oil- degrading microorganisms from soil contaminated with petroleum hydrocarbons Mohsen Soleimani,M. A. Hajabbasi1, Hossein Mirdamadian and Arash Ansari2 1- Isfahan University of Technology, college of agriculture, soil science department, Isfahan, 84155,

(PDF) Isolation and identification of petroleum degrading. MEOR offers major advantages over conventional EOR, namely low energy consumption and independence of the price of crude oil. In this work, the isolation and identification of microorganisms capable of producing biosurfactants and promote degradation of long-chain n-alkanes under conditions existent in oil reservoirs were addressed., Isolation and characterisation of crude oil sludge degrading bacteria Linda U. Obi1,3, Harrison I. Atagana2 and Rasheed A. Adeleke1,3,4* Background High demand for petroleum products all over the world has led to an increase in crude oil extraction and processing. This has resulted in an increased generation of large.

Isolation Optimization and Molecular Characterization of

isolation of microorganisms in oil pdf

Isolation and characterization of bacteria from soil. pdf. Isolation and The population of microorganisms found in oil-polluted environment should degrade petroleum and higher rates than microorganisms in relatively clean environment [9]. we used soil and water samples from different oil contaminated sites for isolation and identification of petroleum degrading bacteria., Isolation and characterisation of crude oil sludge degrading bacteria Linda U. Obi1,3, Harrison I. Atagana2 and Rasheed A. Adeleke1,3,4* Background High demand for petroleum products all over the world has led to an increase in crude oil extraction and processing. This has resulted in an increased generation of large.

Isolation and characterization of phenol degrading

isolation of microorganisms in oil pdf

Isolation Identification and Characterization of Oil. Microorganisms and Crude Oil 115 Fig. 3. Structures of phen ol, furan, and cyclohexanol. Porphyrins often occur in crude oil. They are produc ts of degradation of dyes produced by living organisms. They are composed of pyrrole rings connected by methine bridges. They often form chelate compounds of nickel, vanadium, and other metals. 15.04.2015В В· Yet the diversity of the microorganisms involved in reduction of crystallized ferric irons and the mechanisms underlying the reduction process are largely unknown. A limited number of isolates makes it difficult to understand the physiology and ecological impact of the microorganisms..

isolation of microorganisms in oil pdf


Isolation and identification of oil degrading bacteria from oil sludge in Abadan oil refinery Amin Afifi1, Hossein Motamedi1*, Bahram Alizadeh2, Hadi Leilavi1 1Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran 2Department of Geology, Faculty of Earth Science,Shahid Chamran University of Ahvaz, Ahvaz, Iran Isolation of Microorganism from Natural Source for Bioremediation of Oil-spill *Susant Kumar Padhi 1, Alok kumar Meher2, Lopa pattanaik3, Srikant Tripathy1, BijyalaxmiBehera1 and Jasmin Mishra1 1Division of Biotechnology, MITS Engineering College, Rayagada, Odisha, 765017

Isolation, Optimization, and Molecular Characterization of a produced by microorganisms with the extracellular lipase being produced by many bacteria including Pseudomonas. The Keywords: KY288051, Oil contamination, Pseudomonas spp., 16S rRNA. The present investigation aims to isolate petroleum tolerant and degrading microbes from oil contaminated soil of oil refinery, which later be used in environmental biotechnology as remediating mechanisms. Bushnell Haas Media (BHM) supplemented with diesel oil as carbon and energy source, used for isolation of oil degrading bacteria.

Oil contaminated Sea water, sediment and Soil sample (500g) collected from Oil Jetty, Paradip Port (Orissa) were used for isolation of hydrocarbon utilizing microorganisms. The samples were collected in pre-sterilized sample bottle following aseptic conditions. The samples duly … ADVERTISEMENTS: In this article we will discuss about the isolation and culture of microorganisms. Isolation of Microorganisms: Microorganisms occur in natural environment like soil. They are mixed with several other forms of life. Many microbes are pathogenic. They cause a number of diseases with a variety of symptoms, depending on how they

Isolation of Oil Degrading Microorganisms This was carried out using Minimal Salt Oil Agar. Aliquots (0.1ml) of soil sample suspension were inoculated on Minimal Salt Oil Agar by spread plate method and incubated at room temperature until colonies developed. Isolation of oil- degrading microorganisms from soil contaminated with petroleum hydrocarbons Mohsen Soleimani,M. A. Hajabbasi1, Hossein Mirdamadian and Arash Ansari2 1- Isfahan University of Technology, college of agriculture, soil science department, Isfahan, 84155,

05.05.2015 · Reduction of crystalline Fe(III) oxides is one of the most important electron sinks for organic compound oxidation in natural environments. Yet the limited number of isolates makes it difficult to understand the physiology and ecological impact of the microorganisms involved. Here, two-stage cultivation was implemented to selectively ADVERTISEMENTS: Read this article to learn about the techniques, schemes and strains used for isolating microorganisms. Isolation of Microorganisms: There are over a million species of micro­organisms widely distributed in nature. Less than 1% of the world’s microorganisms have been studied. In fact, only a few hundred species are important

Microorganisms are therefore useful hi protecting the environment. It is sig-nificant to isolate microbes of high potential for the biodegradation of vegetable oil. Therefore, this study was undertaken to isolate, identify, characterize and screen microorganisms from vegetable oil contaminated soil samples. 2. Materials and Methods 2.1. ISOLATION AND SCREENING OF BIOSURFACTANT- PRODUCING BACTERIA FROM SOIL CONTAMINATED WITH DOMESTIC WASTE WATER. *Femi-Ola, T. O., Oluwole, O. A., Olowomofe, T. O. and Yakubu, H. Biosurfactant producing microorganisms were naturally present in oil contaminated soil.

ADVERTISEMENTS: Read this article to learn about the techniques, schemes and strains used for isolating microorganisms. Isolation of Microorganisms: There are over a million species of micro­organisms widely distributed in nature. Less than 1% of the world’s microorganisms have been studied. In fact, only a few hundred species are important ADVERTISEMENTS: Read this article to learn about the techniques, schemes and strains used for isolating microorganisms. Isolation of Microorganisms: There are over a million species of micro­organisms widely distributed in nature. Less than 1% of the world’s microorganisms have been studied. In fact, only a few hundred species are important

Different agar media can be used for the isolation of Actinomycetes from soil sample after dilution of the soil slurry. Actinomycetes Isolation Agar is a selective medium commonly employed. It contains sodium caseinate as nitrogen source. Asparagine in addition to being an amino acid is also a source of nitrogen. isolation of crude oil tolerant microorganisms and his capacity of degradation of crude oil in contaminated soils, such bacteria, yeast and fungi. Therefore, in this work, we isolate different microorganisms, which grow in presence of petroleum as the sole carbon source and we

Different agar media can be used for the isolation of Actinomycetes from soil sample after dilution of the soil slurry. Actinomycetes Isolation Agar is a selective medium commonly employed. It contains sodium caseinate as nitrogen source. Asparagine in addition to being an amino acid is also a source of nitrogen. ADVERTISEMENTS: Read this article to learn about the techniques, schemes and strains used for isolating microorganisms. Isolation of Microorganisms: There are over a million species of micro­organisms widely distributed in nature. Less than 1% of the world’s microorganisms have been studied. In fact, only a few hundred species are important

Isolation and characterisation of crude oil sludge degrading bacteria Linda U. Obi1,3, Harrison I. Atagana2 and Rasheed A. Adeleke1,3,4* Background High demand for petroleum products all over the world has led to an increase in crude oil extraction and processing. This has resulted in an increased generation of large isolation of crude oil tolerant microorganisms and his capacity of degradation of crude oil in contaminated soils, such bacteria, yeast and fungi. Therefore, in this work, we isolate different microorganisms, which grow in presence of petroleum as the sole carbon source and we

View all posts in Cavite category